Restriction Digest of Plasmid DNA
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addgene.org
Restriction Digest of Plasmid DNA
Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA
Oct 11, 2016
Restriction Digest Tutorial
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following restriction map f pglo for the five restriction digests predict consider the the number and lengths of the dna fragments that each digestion of the pglo plasmid will produce digest 87636
following restriction map f pglo for the five restriction digests predict consider the the number and lengths of the dna fragments that each digestion of the pglo plasmid will produce digest 87636
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numerade.com
SOLVED: You have performed a restriction digest on a linear 10.5 Kbp piece of DNA. The sizes of the DNA fragments resulting from the different restriction digests are shown below. Using this information, construct a linear map of this DNA illustrating the location(s) of the different restriction enzyme sites and the distances between each site. (20 pts)Enzyme Size of Fragments Produced (Kbp)BamHI 6.5, 2.5, 1.5EcoRI 5.5, 3.0, 2.0SalI 10.0, 0.5HindIII 8.5, 2.0BamHI/EcoRI 4.0, 2.5, 2.0, 1.5, 0.5Bam
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