You might have noticed that some gas stations occasionally offer specials on unleaded 88 octane gas—also known as E15—which contains 15 percent ethanol. Ethanol is an alcohol that’s derived from corn.

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1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an interaction is ...