The answer depends on the questions you’re trying to answer, as well as the origin, quantity, and quality of your samples. In this article, we’ll briefly discuss several RNA-seq workflows, how they ...
To obtain high-quality, purified nucleic acids, scientists can use several different methods, including phenol-chloroform extraction, cesium chloride/ethidium bromide (CsCl/EtBr) gradient ...
Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...
Transcriptomics represents a critical discipline in cancer research, enabling comprehensive mapping of gene expression profiles and the identification of fusion genes implicated in tumor development.