When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Standard PCR systems require users to set a predefined number of cycles relative to the assay and the input amount. This requires quantifying input material and splitting samples of varying inputs ...