PCR amplification can seem straightforward, but in single-cell workflows even small miscalculations can quickly complicate results and downstream analysis. Conventional PCR systems require users to ...
PCR cycle selection is a delicate balancing act. When preparing RNA-seq libraries, choosing too many cycles risks overcycling, which can compromise data quality and distort transcript representation.
NanoPCR used the plasmonic effect of MPN to perform the RT-PCR process for amplifying coronavirus genes in 11 minutes and then detected the amplified genes within 3 minutes using a magnetic field. To ...
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