In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from ...

Understand the physics behind total internal reflection and why light can become completely trapped inside a medium. This video explains the conditions required for total internal reflection, the role ...

TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...

Bound states in the continuum (BICs) are a special class of resonant states. Although they have the same momentum and energy as the propagating modes in free space, yet they are not coupled to the ...